FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14852730
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG165760.11160237882194048No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG154230.10383949617343075No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC153770.10352978879976946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16450.058.93544843
CTCGTAT15850.056.30845642
GTATGCC17250.054.5790445
TATGCCG17900.052.59712246
CACGTCT23650.051.5009514
ACGTCTG24500.049.4281415
ACACGTC25150.048.4298113
CTGAACT26150.047.11223619
ATGCCGT19350.046.4852247
TCAGATT30700.046.0607072
CACCCAA27300.045.00096531
GCCGTCT16500.044.12052549
GAACTCC28500.043.22739421
GAGCACA29650.041.9067469
TTGAACG33550.041.6256147
CAGATTG34100.041.570793
TGCCGTC19900.040.62764748
CGTATGC23550.040.126944
AGATTGA36900.038.7009284
TATCTCG17450.031.691765
Produced by FastQC (version 0.11.5)