FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12288575
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG130100.10587069696852563No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT128420.10450357344118419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT29200.054.178614
ACGTCTG29200.053.81857315
ACACGTC30150.052.35667413
CTGAACT31400.049.7126419
CATTTTA32750.046.06114234
GAGCACA34600.045.925679
ATTTTAT32550.045.483235
GAACTCC35100.044.27285421
TCAGATT23900.040.5664022
TTGAACG26100.037.8167657
TTATCGC9800.037.4991438
CAGATTG27000.036.9458543
AGTCACC50400.035.2789828
AGATTGA28850.034.9406784
CAGTCAC55350.032.7563127
TGTAAAA11450.032.09533762
TTATCGG12250.030.2850238
CACCATT52150.029.73170931
TGTTTAC34350.029.6515455
ACCATTT52100.029.55870632
Produced by FastQC (version 0.11.5)