FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10931969
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG127610.11673102988125927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT29250.057.55555314
ACGTCTG29850.056.3981415
ACACGTC30050.055.90682213
CTGAACT32200.051.62972319
GAGCACA33550.050.388639
GAACTCC33850.048.5960821
CATGGCA33000.047.9400534
GTCTGAA47400.036.2549417
AGTCACC50250.035.73248328
CAGTCAC53600.034.21719727
TCAGATT18500.033.298212
CAGATTG19500.031.411263
TTGAACG19200.031.3548837
AGCACAC54800.031.10458210
AACTCCA59300.029.39264922
TCTGAAC57800.029.30782318
TATCTCG13400.028.471455
AGATTGA22250.027.8437144
GCACACG62550.027.3623611
CCATGGC62950.026.2990133
Produced by FastQC (version 0.11.5)