FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198895
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG175880.15705120907018058No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA143050.1277358167926389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGATT31900.046.741852
ACGTCTG15850.046.5930115
CACGTCT16050.046.23068614
ACACGTC16150.045.9448413
TTGAACG33600.044.0636377
CAGATTG34450.043.1804053
AGATTGA36400.041.8287474
CTGAACT17800.040.89846819
GAACTCC18950.038.41633621
GAGCACA19850.037.7338039
GATTGAA44450.034.017235
CTTGTCT18550.030.3799061
AACGCTG51050.030.03002510
GTATGCC5500.029.27256645
CAGGCCT50600.029.2584721
TGTTTAC23050.029.1552855
TATGCCG5100.028.13709846
CTCAGAT59850.027.1367781
CCTAACA55300.027.02553225
TATCTCG11650.026.7393655
Produced by FastQC (version 0.11.5)