FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13079460
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG215460.16473157148689627No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA158400.12110591721676583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAAT25350.055.22620435
TCGTATG10850.054.51572443
CACGTCT28150.052.84172414
CCACTCA28000.052.25000833
CTCGTAT11000.052.18142742
ACGTCTG28600.052.1326815
GTATGCC12550.051.87192545
CTCAATC23100.050.4539836
ACACGTC30000.049.69982513
CTGAACT30400.049.6213419
GAACTCC30650.048.75964721
TATGCCG12850.048.2095746
CACCACT31950.046.55730431
TCAGATT33450.046.459012
GAGCACA33050.045.537259
TTGAACG36550.042.5172737
CAGATTG37700.041.8714753
AGATTGA38550.041.2206234
TCAATCT22350.040.40207337
ATGCCGT13200.040.3027347
Produced by FastQC (version 0.11.5)