FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18549052
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT270550.14585651061844024No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG203220.10955815962993688No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG193390.10425869742561507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT28600.055.92642235
TATGCCG16700.051.97562846
CTCGTAT17950.050.30642
TCGTATG17400.048.87891843
GTATGCC18350.048.44647645
CGATATC31200.048.3492336
CACGTCT35150.046.60040714
ACGTCTG35650.045.75022515
ACACGTC37150.044.46885313
ACCACGA36700.044.44177632
CTGAACT38750.042.27028319
CCACGAT38950.042.05414233
TCAGATT35000.041.6014562
ATGCCGT19800.041.3635247
GAACTCC40400.040.63064221
TTGAACG35700.040.3923727
CAGATTG36650.039.4420363
GAGCACA44100.037.698929
CTTGTCT36250.037.560241
AGATTGA39150.037.5491074
Produced by FastQC (version 0.11.5)