FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-ZmARR2U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-ZmARR2U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12843142
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT559770.43585128934959994TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA254340.19803565202346904No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT201070.1565582627677869No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA178510.1389924677310272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC79450.055.50571446
TCGTATG80350.054.97110745
CTCGTAT80350.053.44655244
TATGCCG83750.052.73903748
AGTCACG87700.050.56636428
GCCTTAT86450.049.91977737
TATCTCG84750.049.8045141
GTATGCC89600.049.4134647
TTATCTC92900.045.62341340
TCACGTG97550.045.42407230
GGCCTTA97450.044.93123236
CTTATCT94500.044.74071539
TCTCGTA94850.044.64872743
CCTTATC95800.044.1339338
ACGTCTG104600.043.567315
CAGTCAC103150.043.09440627
GTCACGT106000.041.80349329
CACGTGG106350.041.6326831
CACGTCT110550.041.41206714
GTCTGAA111700.040.76510617
Produced by FastQC (version 0.11.5)