FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-ZmARR2U2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-ZmARR2U2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498747
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC252050.3878439951578358TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA137870.21214858802781522No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA69520.10697446753966573No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT66980.10306602180389544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG36450.053.9650343
TATGCCG36550.053.9114846
CTCGTAT34550.053.28577842
CGTATGC37400.053.2493544
GTATGCC40800.048.81115345
CAGTCAC46350.043.79977827
AGTCACC46550.043.53640428
ACGTCTG47800.042.90751315
CACGTCT50350.040.59541314
TCTCGTA44000.040.48915541
GTCTGAA51400.040.17437717
CCAGTCA53950.037.759726
CGTCTGA55150.037.18910216
CCGTCTT54050.036.77981650
AAAAGGG51500.036.15413770
AGGCGAT53950.035.6175935
TCTGAAC58400.035.2987518
TGAAAAA57350.035.21378362
AGCACAC60050.034.85446510
CACACGT60200.034.53479812
Produced by FastQC (version 0.11.5)