FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-ZmARR2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-ZmARR2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476400
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT681150.6501756328509793TruSeq Adapter, Index 27 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA331150.3160914054446184No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT173610.16571532205719522No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA160210.15292466877935168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC88550.058.85340546
CTCGTAT87100.058.7881244
TATGCCG89900.057.6965548
TCGTATG90550.057.55322645
GTATGCC92650.056.21121247
TATCTCG92000.054.89615241
TCTCGTA99250.050.95663543
TTATCTC99800.050.53577440
GTCACAT105300.050.1915529
ACATTCC104450.050.06121432
AGTCACA105800.049.8220228
CCTTTAT103400.049.79201537
CTTTATC103100.048.9182438
CAGTCAC110000.047.98336427
ACGTCTG114350.046.8609115
CACATTC112600.046.595231
TCCTTTA113850.046.0208136
TCACATT115200.045.7868530
CACGTCT117850.045.52837814
CCGTCTT114450.045.47330552
Produced by FastQC (version 0.11.5)