FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-ZmARR2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-ZmARR2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908264
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC475890.4802960437872871TruSeq Adapter, Index 3 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA150180.1515704466493828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC64850.054.24010544
TCGTATG64950.054.21048443
TATGCCG65550.053.6611546
CTCGTAT65150.053.02361742
TATCTCG68000.049.97702439
GTATGCC70600.049.87210545
ACCTAGC72800.048.65442732
CACCTAG74000.047.9122531
TCTCGTA76500.044.65299641
AGCTATC76850.044.6324836
AGTCACC79600.044.452728
TAGCTAT78700.044.25061835
GCTATCT77550.044.0490837
CCTAGCT82050.043.2546333
ACGTCTG83250.043.09404415
CAGTCAC82850.042.62444327
CTATCTC80950.042.1987538
CACGTCT87100.041.4702814
CGTCTGA89400.040.05040716
AAAAGGG83450.039.551370
Produced by FastQC (version 0.11.5)