FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-6597U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-6597U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17852474
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1207530.6763936471774163TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA373730.20934353412305767No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA293000.16412291091979747No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT292970.1641061065261879No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG207790.11639283160417992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC159100.059.50568446
TATGCCG159550.059.0521848
TCGTATG160000.058.90846345
CTCGTAT158900.057.90659744
AGTCACG169600.056.42280628
GTATGCC169700.055.9331447
ACGTCCG181050.052.81406432
TCTCGTA178500.050.97960343
TCCGCAC187100.050.93821735
GTCACGT187850.050.84805329
CAGTCAC191300.050.1874727
ACGTCTG194900.049.79823715
CGCACAT190250.048.97247337
CCGCACA194500.048.6041836
CACGTCT200350.048.4085314
GTCCGCA197400.048.38699334
GTCTGAA206050.047.25505417
TCACGTC204650.046.7421530
CCGTCTT202150.046.6073252
ATGCCGT206250.045.88458349
Produced by FastQC (version 0.11.5)