HW2NGBGX5_n01_ZmTARGET.0523-6597U2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-6597U2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8228061
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC	32773	0.3983076936352319	TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	19792	0.2405426989420715	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	16225	0.19719105145185484	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	10728	0.1303830878259167	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	4865	55.68276	44
TCGTATG	4955	54.529438	43
TATGCCG	5035	53.455143	46
CTCGTAT	4980	51.93679	42
GTATGCC	5315	51.034172	45
ACTCAAT	5740	46.525223	35
ACGTCTG	6130	45.907322	15
AGTCACC	6220	44.00454	28
CACGTCT	6405	43.936283	14
CCACTCA	6210	43.849155	33
CGTCTGA	6580	42.712753	16
TCTCGTA	6045	42.439507	41
GTCTGAA	6685	42.35601	17
CAGTCAC	6505	42.292076	27
ACCACTC	6605	41.38505	32
CACTCAA	6610	41.089745	34
CTCAATC	6240	40.88966	36
CACACGT	7025	40.506805	12
AATCTCG	6355	40.204308	39
AAAAGGG	6455	38.442123	70