HW2NGBGX5_n01_ZmTARGET.0523-6597S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-6597S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10177963
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT	151401	1.487537339249514	TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	39657	0.38963592223709204	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	21609	0.21231163838972494	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	20381	0.2002463557786563	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	13401	0.13166681781020426	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	11278	0.11080802710719227	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	11141	0.10946198173445905	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	10453	0.10270227942467465	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	17390	64.18276	46
CTCGTAT	17390	63.33746	44
TATGCCG	17605	63.27965	48
TCGTATG	17700	63.11798	45
GTATGCC	17965	61.99211	47
TATCTCG	17875	61.658398	41
GTCACAC	18750	60.218033	29
TCTCGTA	18535	59.556965	43
ACTGATA	19010	59.41342	34
AGTCACA	19140	59.045876	28
TATATCT	18795	58.715332	39
TCACACT	19345	58.25733	30
CAGTCAC	19545	57.804455	27
ATATCTC	19105	57.780643	40
TGATATA	19525	57.739037	36
CCGTCTT	19490	57.05089	52
GATATAT	19845	56.843838	37
CTGATAT	19900	56.809284	35
CACACTG	19885	56.640366	31
ACGTCTG	20265	56.616203	15