HW2NGBGX5_n01_ZmTARGET.0523-6435U.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-6435U.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10657195
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC	123902	1.1626136145580521	TruSeq Adapter, Index 3 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	43112	0.404534213740107	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	31809	0.29847441094959787	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	30158	0.28298252964311904	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	15385	0.14436256444589782	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	13864	0.13009051631315743	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	14930	61.770634	44
TATGCCG	14910	61.736122	46
CTCGTAT	14650	61.350002	42
TCGTATG	15055	61.326927	43
GTATGCC	15390	59.74241	45
AGTCACG	15615	59.400433	28
ACGTAGA	16025	57.92218	32
CGTAGAG	16210	57.30458	33
GTCACGT	16420	56.44566	29
TAGAGAT	16285	56.26642	35
TCTCGTA	15915	56.209717	41
GTAGAGA	16785	54.96617	34
CAGTCAC	16960	54.772274	27
ACGTCTG	17345	54.15987	15
CACGTCT	17555	53.571552	14
CCGTCTT	17165	53.461857	50
AAAAGGG	15950	53.212337	70
ATGCCGT	17585	52.444202	47
GTCTGAA	17915	52.435684	17
GCTTGAA	17690	51.99857	59