HW2NGBGX5_n01_ZmTARGET.0523-6435S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-6435S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8907131
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC	88962	0.9987727810447606	TruSeq Adapter, Index 22 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	27071	0.3039250236692376	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	14401	0.16167944537921358	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	14024	0.1574468816053115	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	9401	0.10554464731685209	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	10895	62.257195	44
TCGTATG	11080	61.05976	43
TATGCCG	11080	60.99727	46
CTCGTAT	10815	60.970165	42
GTATGCC	11400	59.315434	45
AGTCACG	11595	58.893726	28
GGTAGCA	12375	54.98241	34
TAGCATC	11880	54.974724	36
CGGTAGC	12425	54.92986	33
GTAGCAT	12230	54.775425	35
ACGGTAG	12500	54.572594	32
CAGTCAC	12645	53.9757	27
TCTCGTA	12055	53.91446	41
ACGTCTG	13075	53.108665	15
CACGGTA	12845	53.108635	31
AAAAGGG	11830	52.633114	70
CACGTCT	13220	52.57911	14
CCGTCTT	13045	51.459732	50
GTCTGAA	13640	50.984905	17
ATGCCGT	13315	50.705948	47