FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3357U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3357U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19983152
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC587770.29413277745172534TruSeq Adapter, Index 16 (98% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG306140.15319905488383412No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC242670.12143729878049268No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT201780.10097506139171639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC98050.050.86591344
TCGTATG99150.049.48958243
TATGCCG100000.049.27940846
CTCGTAT94200.048.82070542
GTATGCC105500.047.1747945
AGTCACC129000.038.85331728
TCTCGTA113700.038.6317141
CAGTCAC134250.037.69900527
ACGTCTG143100.036.6147315
CACGTCT146550.035.7049114
GTCTGAA148650.035.08172617
CCGTCTT144950.033.82824350
AAAAGGG135950.033.7006770
TGAAAAA150700.033.35196362
CGTCCAT141400.033.2427635
TCACCCG153450.032.66277730
ACCCGTC157300.031.90735832
CCAGTCA160950.031.83654826
CACCCGT157550.031.7462131
GCTTGAA157700.031.64998259
Produced by FastQC (version 0.11.5)