HW2NGBGX5_n01_ZmTARGET.0523-3357S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-3357S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8922267
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT	111422	1.2488081784595775	TruSeq Adapter, Index 19 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	22133	0.24806475753303506	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	13421	0.15042141195729739	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	11830	0.13258962100103033	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	11049	0.12383624027391243	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	10956	0.12279390428463977	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	10774	0.120754063961547	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	13880	63.318314	46
CTCGTAT	13240	63.179993	44
TCGTATG	13910	63.131428	45
TATGCCG	13975	62.760548	48
GTATGCC	14285	61.52246	47
AGTCACG	14625	60.33286	28
TCTCGTA	14065	58.92696	43
ACGTGAT	15025	58.725674	32
AATCTCG	14135	58.38785	41
CACGTGA	15135	58.23079	31
TCACGTG	15195	57.954464	30
GTCACGT	15390	57.220146	29
CAGTCAC	15560	56.662792	27
CCAATCT	14690	56.15714	39
CCGTCTT	16010	54.783176	52
GCTTGAA	16095	54.60473	61
ACGTCTG	16415	54.222553	15
ATGCCGT	16270	53.907722	49
CAATCTC	15410	53.64778	40
GCCGTCT	16360	53.632557	51