FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3357S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3357S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4716093
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1376802.919365669845781TruSeq Adapter, Index 2 (97% over 36bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC85880.18209988649502884No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT59320.1257820827536692No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG53540.11352617516236427No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG52650.11163901984121177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC161450.066.83559444
CTCGTAT155550.066.6698242
TCGTATG163100.065.9012443
TATGCCG163900.065.77106546
GCCGTCT164650.065.1731849
ACGTCTG168750.064.9813815
GTATGCC166000.064.8553745
CACGTCT169250.064.6653414
AGTCACC168350.064.2210128
CAGTCAC169150.064.18626427
ATGCCGT168650.063.7733647
CCGTCTT170550.063.0211950
ACACGTC174350.062.99460213
GCTTGAA170650.062.90489259
GTCTGAA174600.062.7627517
TCTCGTA165050.062.2810841
CACCCAA174150.062.06402631
CCAGTCA175400.061.93903726
CAATCTC166100.061.65558638
TGCTTGA173750.061.58111658
Produced by FastQC (version 0.11.5)