FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3191U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3191U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21177365
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT673500.3180282343908225TruSeq Adapter, Index 15 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG319240.1507458553035281No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC230310.10875290670014895No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT229530.10838458892312618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC101050.051.2613846
TATGCCG102500.050.8092748
TCGTATG103650.049.5027845
CTCGTAT102200.049.04073744
GTATGCC114000.045.96019747
TCTCGTA124350.040.67062443
AGTCACA137750.039.81572328
GTCACAT138100.039.46127729
AATCTCG136800.037.14850641
ACGTCTG148800.037.04680315
CAGTCAC149600.036.7320627
TCACATG153300.035.57127430
CACATGT154800.035.33997331
ATGTCAG156850.035.3237234
CACGTCT156650.035.19024314
GAATCTC149450.034.12121240
GTCTGAA164150.033.9868817
CGTCTGA163000.033.96899816
CCGTCTT151250.033.9464652
AGAATCT152950.033.68396839
Produced by FastQC (version 0.11.5)