FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3191U2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3191U2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12536525
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC1105380.8817275919762455TruSeq Adapter, Index 23 (97% over 38bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA269590.21504364247668314No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT129330.10316255900259441No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA128630.1026041905551977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC154550.059.2198444
TCGTATG154100.059.16611543
TATGCCG154600.058.88350746
CTCGTAT149800.057.75742
GTATGCC161750.056.45394545
ACTAATC153100.055.82647336
CTAATCT153350.055.59896537
TAATCTC158350.053.75433338
AACTAAT171650.052.64803335
CAACTAA174600.052.4605334
AGTCACC176000.052.24292828
ACGTCTG180050.052.03956215
TCTCGTA165050.051.8689241
AATCTCG165150.051.5192139
CACGTCT182050.051.42899314
CAGTCAC182300.050.51429427
GTCTGAA187100.050.11410517
CGTCTGA187550.049.9378816
CCAACTA186550.049.3251633
AAAAGGG172750.049.2322170
Produced by FastQC (version 0.11.5)