FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3191S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3191S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10114479
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT3681273.639604175360886TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA1309191.294372156984062No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT661740.6542502090320224No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA658300.6508491440834472No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG328870.3251477411738163No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA307390.3039108588786432No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA199980.19771656058606676No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA189570.18742438438994236No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG165900.1640222892350659No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC152860.1511298802439552No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT149100.14741243716062882No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT147880.14620624552188996No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG128550.12709502881957638No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT110490.10923943783955653No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG109130.10789483076686403No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG108530.10730162176420555No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG103910.10273391244373536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA424600.066.924536
CGTAATC407600.066.8821338
CGTATGC424050.066.8785846
CTCGTAT410100.066.840444
TATGCCG424500.066.5768348
TCGTATG425350.066.5257445
GTATGCC430100.065.8076347
TAATCTC418550.064.8638240
GCCGTCT434000.064.81241651
CCGTACG439700.064.77807633
ACGTAAT434850.064.76781537
TCTCGTA423650.064.3139843
CCGTCTT438350.064.24110452
ATGCCGT440100.064.2407749
GTAATCT423250.064.18552439
CACCGTA443950.064.1450431
AGTCACC444500.064.1223728
ACCGTAC445950.064.034432
CAGTCAC446000.063.96133427
GCTTGAA442800.063.84525361
Produced by FastQC (version 0.11.5)