FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0523-3191S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0523-3191S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845553
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC573490.48413949099717TruSeq Adapter, Index 9 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA379810.32063509403064594No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA225020.18996158305146243No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT210510.17771226045757424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC76800.054.96031644
TCGTATG78300.053.68371243
TATGCCG78900.053.4085546
CTCGTAT78100.052.9249242
TATCTCG81200.050.38693639
GTATGCC85350.049.78247545
TTATCTC90650.045.05740738
CATTTTA95200.044.85300434
AGTCACC95900.044.56304628
TCTCGTA92700.044.173741
ACGTCTG99750.043.82591615
CACGTCT102850.042.675314
CAGTCAC103150.041.39695727
CCGTCTT103400.040.48259450
TTTTATC103800.040.12495836
ATGCCGT105400.040.04698647
AAAAGGG100650.039.29480470
GTCTGAA111750.039.2429117
GCCGTCT106900.038.99361849
ACCATTT109950.038.8993932
Produced by FastQC (version 0.11.5)