HW2NGBGX5_n01_ZmTARGET.0523-183U2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-183U2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23625654
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC	1434464	6.071637212667214	TruSeq Adapter, Index 7 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	197868	0.8375133234406971	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	78096	0.3305559287374648	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	48533	0.20542500114494183	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	33485	0.14173152624685012	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	25210	0.10670604081478548	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	167840	65.82204	43
CGTATGC	168070	65.79596	44
CTCGTAT	163220	65.78757	42
TATGCCG	167935	65.72157	46
GTATGCC	169290	65.23895	45
TCTCGTA	164780	64.57626	41
AGTCACC	173720	63.881977	28
AAAAGGG	161030	63.86586	70
CAGTCAC	174775	63.618385	27
CCGTCTT	172965	63.586803	50
GCCGTCT	173450	63.384785	49
ATGCCGT	174455	63.249275	47
GCTTGAA	174640	63.216354	59
TGAAAAA	176815	62.988907	62
CCAGTCA	177445	62.694527	26
TGCTTGA	175995	62.63021	58
ACGTCTG	179645	62.58605	15
AGATCTC	169110	62.47943	38
TGCCGTC	176730	62.3931	48
GTCTGAA	180240	62.381348	17