HW2NGBGX5_n01_ZmTARGET.0523-183S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0523-183S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10625539
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT	368761	3.470515707485521	TruSeq Adapter, Index 21 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	70614	0.6645686397650039	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	40792	0.3839052305958314	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	33904	0.3190802838331307	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	19953	0.18778341503428675	No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG	17493	0.1646316483333222	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13944	0.1312309897879063	No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA	12617	0.11874221157157298	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	41215	66.28824	44
CGTATGC	43250	66.17089	46
CGTTTCG	46325	65.89828	33
TATGCCG	43405	65.8859	48
TCGTATG	42950	65.83481	45
TTTCGGA	46510	65.67348	35
ACGTTTC	46705	65.421455	32
CACGTTT	46805	65.32122	31
TTCGGAA	46665	65.252525	36
GTATGCC	44185	65.04788	47
AGTCACG	46985	65.04026	28
TCGGAAT	46500	64.91985	37
GTCACGT	47410	64.413826	29
GTTTCGG	47900	63.80485	34
TCTCGTA	42895	63.708344	43
CAGTCAC	48040	63.553627	27
CCGTCTT	43965	63.06448	52
TCACGTT	48555	62.95223	30
ATGCCGT	45205	62.93725	49
ACGTCTG	49085	62.933872	15