FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-ZmARR2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-ZmARR2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18728145
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT360850.19267791871538797TruSeq Adapter, Index 13 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG257610.1375523309970101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63800.045.58720445
CGTATGC65050.044.4962246
TATGCCG65800.043.8292448
CTCGTAT65300.042.98567644
GTATGCC76150.038.1483247
TCTCGTA85700.032.83497243
AGTCACA97350.030.74044228
CACAGTC98900.030.25858531
ACGTCTG102900.029.42218415
ACAGTCA105250.028.46527532
CAGTCAC106200.028.24465827
ACTCTAG80700.027.3668125
AATCTCG103250.027.32154341
CACGTCT110550.027.16441714
AGTCAAC113950.026.6914834
GTCTGAA114750.026.41368317
CGTCTGA116400.026.15960716
TCACAGT117000.025.7570530
CCGTCTT114300.025.62939552
CACACGT119500.025.56926312
Produced by FastQC (version 0.11.5)