FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-6597U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-6597U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30584722
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG581860.19024531267604786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC504140.16483393244509464TruSeq Adapter, Index 5 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC489230.15995894943887343No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT374410.12241732980276886No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG329380.10769429259484523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90600.045.2756944
TATGCCG92100.044.7661746
TCGTATG91450.044.7396843
CTCGTAT87350.043.3537342
GTATGCC104550.039.33503345
GTCACAC128900.032.61149229
AGTCACA137200.030.6385928
TCTCGTA127600.029.321741
ACTCTAG148900.029.3121285
CACAGTG143800.029.085933
AGTGATC132650.028.52259636
GACTCTA104250.028.4362494
AAAAGGG136350.028.41573370
ACGTCTG155050.027.6750215
ACAGTGA152250.027.586634
ACACAGT156550.026.73895832
CTGACTC110000.026.6633592
CAGTGAT155250.026.24178935
TGAAAAA160800.026.09821962
GCTGACT113050.026.0375181
Produced by FastQC (version 0.11.5)