HW2NGBGX5_n01_ZmTARGET.0521-6597S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0521-6597S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23138770
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC	58568	0.2531163065279615	TruSeq Adapter, Index 12 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	51699	0.2234301996173522	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	42329	0.1829353937136676	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	31756	0.13724152148104674	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	26523	0.11462579903771894	No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG	26213	0.11328605625968882	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	9365	50.042873	44
TATGCCG	9535	48.819977	46
TCGTATG	9775	47.87207	43
CTCGTAT	9620	46.60608	42
GTATGCC	10650	43.906242	45
GTAATCT	11155	39.722313	37
TAATCTC	11595	38.27499	38
TGTAATC	11730	38.043682	36
AATCTCG	11885	37.458942	39
TCTCGTA	12025	37.197624	41
AGTCACC	13030	36.101887	28
CTTGTAA	13480	35.078537	34
TTGTAAT	13670	34.309036	35
CAGTCAC	13920	33.843937	27
ACGTCTG	14255	33.6383	15
AAAAGGG	13555	32.61105	70
ACTCTAG	12290	32.03807	5
CCGTCTT	15005	31.232346	50
CGTCTGA	15280	31.197811	16
GACTCTA	8570	31.037802	4