HW2NGBGX5_n01_ZmTARGET.0521-6435S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0521-6435S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38245605
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC	122606	0.32057539683317865	TruSeq Adapter, Index 10 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	74135	0.1938392659757899	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	56415	0.14750714493861453	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	53208	0.13912186772833113	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	39089	0.10220520763104676	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	17905	53.207947	44
TATGCCG	18190	52.605453	46
TCGTATG	18420	51.54924	43
CTCGTAT	18740	50.108704	42
GTATGCC	20585	46.55274	45
TATCTCG	20485	45.635204	39
AGTCACT	23390	42.348183	28
GTCACTA	24225	41.018482	29
TCTCGTA	23010	40.870533	41
TTATCTC	23160	40.697193	38
TCACTAG	24400	40.53771	30
GCTTATC	23790	40.45794	36
CTTATCT	23765	40.058834	37
ACGTCTG	25205	39.74287	15
CACGTCT	26610	37.749535	14
TAGCTTA	26245	37.673866	34
CACTAGC	26500	37.377922	31
CAGTCAC	26695	37.21002	27
CCGTCTT	26365	36.041515	50
ATGCCGT	27375	35.05733	47