FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-3357U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-3357U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920782
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC291480.22559006103500548TruSeq Adapter, Index 3 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG212360.16435537725193414No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC163490.12653258912657142No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT133360.10321356710452975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47450.048.9033944
CTCGTAT47450.047.79697842
TATGCCG49000.047.3568146
TCGTATG49750.046.78323443
ACTTAGG52250.044.81412532
GTATGCC52950.043.95608545
CACTTAG58300.040.7045531
TTAGGCA58100.040.30185734
TCACTTA58700.040.2484530
TAGGCAT62900.036.1688335
GTCACTT65350.036.0992229
AGTCACT65450.035.88363328
TCTCGTA64750.034.48584741
AAAAGGG64950.032.92573570
TGAAAAA72950.032.1453362
ACGTCTG77450.031.45269615
CAGTCAC75900.031.26593627
GTCTGAA80200.030.15506717
GCTTGAA77400.029.8905559
AGGCATC75300.029.84089336
Produced by FastQC (version 0.11.5)