HW2NGBGX5_n01_ZmTARGET.0521-3357S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0521-3357S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14591571
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	73374	0.5028519547346889	TruSeq Adapter, Index 9 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	30402	0.20835316498819764	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	20686	0.14176677754574885	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	20472	0.14030017741064343	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	15528	0.10641760232671316	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	9815	57.804047	44
TCGTATG	10205	55.697483	43
CTCGTAT	9970	55.149754	42
TATGCCG	10380	54.55634	46
AGTCACG	10745	53.030464	28
GTATGCC	10815	52.459064	45
GTCACGA	11630	48.904755	29
TCTCGTA	11280	48.403645	41
TCACGAT	12665	45.1844	30
ACGTCTG	13045	44.484985	15
CGTCTGA	13325	43.78541	16
ACGATCA	13245	43.442554	32
CACGATC	13255	43.17332	31
CAGTCAC	13240	43.089905	27
CACGTCT	13525	42.98385	14
AAAAGGG	12190	42.896156	70
ATGCCGT	13500	42.18095	47
GTCTGAA	13845	41.93851	17
CCGTCTT	13605	41.803665	50
CGATCAG	13995	41.03956	33