FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-3191U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-3191U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21743993
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG398680.18335178823871034No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC333280.15327451586284083TruSeq Adapter, Index 2 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC295790.13603297241679577No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT287350.13215144063006273No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG224060.1030445512008765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC61100.042.2171244
TCGTATG60550.042.13827543
TATGCCG61500.041.94273446
CTCGTAT61700.040.4452742
GTATGCC69950.036.67580445
GTATCTC79350.031.75805938
ACTCTAG116400.029.8578595
TATCTCG86950.028.82078639
CTGACTC80650.028.598382
GACTCTA84050.028.440774
TGTATCT89150.028.26718337
ACCGATG94150.028.0297632
GCTGACT85200.027.2768291
CACCGAT97450.026.65007431
ATGTATC96150.026.57320436
TCTCGTA94600.026.49013941
GATGTAT100550.025.82808335
ACGTCTG103350.025.56935915
AAAAGGG93900.025.3835870
CGATGTA107400.024.47422834
Produced by FastQC (version 0.11.5)