FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-3191S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-3191S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9126925
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC169910.18616346688506807TruSeq Adapter, Index 8 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG165190.18099195512179622No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT148080.16224522497993574No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC126990.13913777093599433No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG98860.10831687561802031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30550.043.9927344
TATGCCG31400.043.47062346
TCGTATG31600.042.53141443
CTCGTAT31550.041.71133842
GTATGCC36800.036.99667445
GTCACAC43450.032.62495829
TCTCGTA40150.032.60238341
AATCTCG41950.031.45359839
AGTCACA47650.029.74930628
ACGTCTG49950.029.57038115
CACACTT49650.028.8329331
CAGTCAC49650.028.69193627
CTGACTC43500.028.4018332
TCACACT50150.028.33608430
GCTGACT43050.028.2923741
ACTCTAG56700.027.7777775
CACGTCT53450.027.6992414
GAATCTC48800.027.1820838
GACTCTA46750.027.1010114
CCGTCTT50400.026.8045350
Produced by FastQC (version 0.11.5)