FastQCFastQC Report
Thu 14 Jun 2018
HW2NGBGX5_n01_ZmTARGET.0521-183U.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_ZmTARGET.0521-183U.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17654987
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG283250.16043625520653174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC271970.15404712560819217TruSeq Adapter, Index 1 (100% over 50bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT190000.1076183177025279No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC180970.10250361555066566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49200.042.7535344
TATGCCG49900.041.6629246
TCGTATG50900.041.60030443
CTCGTAT51050.039.6955442
GTATGCC56950.036.5668145
AGTCACA75650.028.5005228
GTCACAT77800.027.93785329
TCTCGTA74750.026.54793541
ACTCTAG84650.026.214165
TCACGAT82300.025.6438835
GACTCTA62650.025.1951454
ACGTCTG88400.025.10220515
CAGTCAC88250.024.58997227
ATCACGA88650.024.51773534
GCTGACT62950.024.1305751
AAAAGGG82500.024.09680470
GTCTGAA93200.023.99661417
TGAAAAA90150.023.72208462
CTGACTC63700.023.5159232
CACGTCT94950.023.33370214
Produced by FastQC (version 0.11.5)