HW2NGBGX5_n01_ZmTARGET.0521-183S.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW2NGBGX5_n01_ZmTARGET.0521-183S.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26364696
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	150841	0.5721325214597581	TruSeq Adapter, Index 7 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	51513	0.19538628474987915	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	42305	0.1604607919620996	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	37600	0.14261495751743164	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	36373	0.1379610066431261	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	27755	0.10527335494405093	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	19935	57.710007	44
TCGTATG	20000	57.312016	43
TATGCCG	20015	57.251904	46
CTCGTAT	19660	56.57611	42
GTATGCC	21780	52.80528	45
TCTCGTA	22390	49.03682	41
AGTCACC	24665	47.836224	28
AAAAGGG	22995	46.194344	70
CAGTCAC	25815	45.759457	27
ACGTCTG	26555	45.616608	15
CACGTCT	27640	43.838516	14
CGTCTGA	27630	43.76505	16
CCGTCTT	26305	43.37542	50
GTCTGAA	28100	43.282143	17
TGAAAAA	27650	42.620945	62
ATGCCGT	26995	42.591183	47
GCTTGAA	27135	42.41094	59
CCAGTCA	28855	41.229614	26
GCCGTCT	27705	41.221687	49
CACACGT	29475	41.180706	12