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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-06-01, 03:06 based on data in: /beegfs/mk5636/logs/html/HVT5JBGX5/merged


        General Statistics

        Showing 22/22 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HVT5JBGX5_n01_03_12_GR_Dam_2
        26.4%
        45%
        17.6
        HVT5JBGX5_n01_03_12_GR_Dam_5
        31.4%
        47%
        20.7
        HVT5JBGX5_n01_03_12_GR_Dam_8
        24.0%
        43%
        20.1
        HVT5JBGX5_n01_03_12_GR_Dam_bZIP1_3
        43.6%
        50%
        21.4
        HVT5JBGX5_n01_03_12_GR_Dam_bZIP1_9
        33.7%
        46%
        24.9
        HVT5JBGX5_n01_03_12_GR_Only_1
        65.8%
        56%
        26.4
        HVT5JBGX5_n01_03_12_GR_Only_4
        67.0%
        56%
        25.9
        HVT5JBGX5_n01_03_26_GR_Dam_11
        43.7%
        55%
        14.8
        HVT5JBGX5_n01_03_26_GR_Dam_2
        54.7%
        56%
        26.4
        HVT5JBGX5_n01_03_26_GR_Dam_5
        47.9%
        54%
        16.5
        HVT5JBGX5_n01_03_26_GR_Dam_8
        36.1%
        52%
        15.3
        HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_12
        56.1%
        56%
        24.4
        HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_3
        48.5%
        57%
        13.0
        HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_6
        47.6%
        57%
        11.7
        HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_9
        53.4%
        58%
        13.9
        HVT5JBGX5_n01_04_09_GR_Dam_2
        70.1%
        56%
        29.1
        HVT5JBGX5_n01_04_09_GR_Dam_5
        70.8%
        57%
        32.7
        HVT5JBGX5_n01_04_09_GR_Dam_8
        66.9%
        56%
        21.6
        HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_3
        65.2%
        55%
        21.9
        HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_6
        62.0%
        55%
        19.0
        HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_9
        63.7%
        55%
        19.2
        HVT5JBGX5_n01_undetermined
        38.4%
        56%
        17.6

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 22/22 rows and 3/3 columns.
        Sample NameTotal Read CountPortion (%)Perfect (%)
        undetermined_library
        17,642,382
        3.9
        -1.0
        03_26_GR_Dam_bZIP1_12
        24,351,382
        5.4
        -1.0
        03_26_GR_Dam_bZIP1_9
        13,908,232
        3.1
        -1.0
        03_26_GR_Dam_bZIP1_6
        11,724,506
        2.6
        -1.0
        03_26_GR_Dam_bZIP1_3
        12,979,058
        2.9
        -1.0
        03_26_GR_Dam_11
        14,761,166
        3.3
        -1.0
        03_26_GR_Dam_8
        15,290,981
        3.4
        -1.0
        03_26_GR_Dam_5
        16,520,934
        3.6
        -1.0
        03_26_GR_Dam_2
        26,360,061
        5.8
        -1.0
        04_09_GR_Dam_bZIP1_9
        19,187,393
        4.2
        -1.0
        04_09_GR_Dam_bZIP1_6
        19,013,897
        4.2
        -1.0
        04_09_GR_Dam_bZIP1_3
        21,852,653
        4.8
        -1.0
        04_09_GR_Dam_8
        21,559,219
        4.8
        -1.0
        04_09_GR_Dam_5
        32,725,184
        7.2
        -1.0
        04_09_GR_Dam_2
        29,094,822
        6.4
        -1.0
        03_12_GR_Dam_bZIP1_9
        24,888,799
        5.5
        -1.0
        03_12_GR_Dam_bZIP1_3
        21,407,448
        4.7
        -1.0
        03_12_GR_Dam_8
        20,066,899
        4.4
        -1.0
        03_12_GR_Dam_5
        20,670,554
        4.6
        -1.0
        03_12_GR_Dam_2
        17,552,026
        3.9
        -1.0
        03_12_GR_Only_4
        25,882,324
        5.7
        -1.0
        03_12_GR_Only_1
        26,366,392
        5.8
        -1.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        453,806,312
        453,806,312
        3.9
        0.7

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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