FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17642382
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178497610.117545351869152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC1752800.067.5050311
AGGATCT578050.066.9057112
GGTCGCG1728950.066.836381
CCGAGGA1783850.066.708799
CGAGGAT1779250.066.610110
AGGATCA604950.066.5876812
CGGCCGA1829850.065.99676
GGCCGAG1832700.065.942247
GTCGCGG1787100.065.58012
AGGATCG220200.065.5537112
GCCGAGG1856600.065.091118
GGATCTT166750.064.6551113
TCGCGGC1817450.064.6543963
CGCGGCC1865850.064.495624
GGATCTA87900.064.4477513
GGATCAA179450.064.4416813
GGATCTC173550.064.4375413
GCGGCCG1901550.063.8743825
GGATCTG177050.063.2426613
GGATCAT180900.062.8626313
Produced by FastQC (version 0.11.5)