FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_bZIP1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187393
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG504940.2631623795895565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2211650.064.28236411
AGGATCT756600.064.2740612
GGTCGCG2231450.063.8618931
AGGATCA675450.063.7524212
CCGAGGA2260900.063.1593369
GGATCTT220300.062.7680113
CGGCCGA2301050.062.655766
CGAGGAT2275650.062.6438410
GGCCGAG2302700.062.6270267
GGATCTA75000.062.20348413
GTCGCGG2296400.062.1842462
GGATCAA173200.061.71162413
GGATCTC244100.061.68057613
GGATCAT201050.061.6755313
GCCGAGG2342950.061.4525538
AGGATCG281050.061.341512
GGATCTG250350.060.72786713
CGCGGCC2365900.060.5878684
GGATCCA174750.060.54339613
TCGCGGC2360100.060.5335773
Produced by FastQC (version 0.11.5)