FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_bZIP1_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19013897
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501500.2637544528615044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2286100.062.56492211
GGTCGCG2290700.062.556551
AGGATCT780750.062.40653612
AGGATCA708750.062.0256912
CCGAGGA2340450.061.4778759
CGGCCGA2360900.061.4107976
CGAGGAT2342100.061.27984610
GGCCGAG2368800.061.2436147
GTCGCGG2350100.061.0697632
GGATCTT233350.060.8474313
GCCGAGG2411550.060.1272968
GGATCAA180650.060.0577113
GGATCAT224450.060.04805813
CGCGGCC2418300.059.631834
TCGCGGC2415750.059.4716263
GGATCTA83550.059.35651813
GGATCTC245700.059.27036313
GGATCCG105700.059.2683813
CTAATAC134700.059.234671
GGATCCA190150.059.04499413
Produced by FastQC (version 0.11.5)