FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_bZIP1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_bZIP1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21852653
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2588500.065.4725211
AGGATCT871950.065.15575412
AGGATCA775550.064.6172512
GGTCGCG2615050.064.5140841
CCGAGGA2639500.064.37389
CGAGGAT2658050.063.9358110
CGGCCGA2681900.063.831016
GGCCGAG2685100.063.7729847
GGATCTT244100.063.25826613
AGGATCG341400.062.78965812
GTCGCGG2697050.062.7615242
GGATCCA223000.062.66773213
GGATCAT239250.062.60965713
GCCGAGG2736750.062.585468
GGATCTA84050.062.501813
GGATCAA187700.062.4640413
GGATCTC301000.062.17177213
GGATCCC142400.061.56689513
CGCGGCC2768050.061.485264
GGATCTG286100.061.3238513
Produced by FastQC (version 0.11.5)