FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21559219
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2906650.066.5315511
AGGATCT976300.066.5048312
AGGATCA863050.066.3710812
GGATCTT287250.065.44054413
CCGAGGA2966150.065.409339
AGGATCG350550.065.3938712
CGAGGAT2975650.065.16521510
GGATCTC312650.065.127913
GGTCGCG2972350.065.014041
GGATCAA206500.064.76028413
GGATCCA247150.064.701213
CGGCCGA3037100.064.636536
GGATCGT94400.064.6215213
GGATCAT268250.064.595913
GGCCGAG3042300.064.569937
GGATCTA96950.064.4380213
GGATCCC156200.064.0821113
GCCGAGG3080050.063.7362028
GGATCTG308400.063.71032713
GGATCCG128450.063.567113
Produced by FastQC (version 0.11.5)