FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32725184
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1464700.067.01173412
GAGGATC4373000.066.73283411
AGGATCA1275400.066.7114712
GGATCTT420500.066.00172413
AGGATCG531500.065.86748512
GGATCTA132100.065.8372513
GGATCTC488400.065.73305513
GGATCCA375750.065.7122213
CCGAGGA4466500.065.602199
CGAGGAT4473050.065.4225110
GGATCAA290200.065.40188613
GGATCCC239350.065.1275713
GGATCAT388200.064.88496413
GGTCGCG4530750.064.8731841
GGATCTG461200.064.5861413
CGGCCGA4637750.064.497826
GGCCGAG4643950.064.41187
GGATCCG194600.063.82813
GCCGAGG4691550.063.7299278
GGATCAC301900.063.55124313
Produced by FastQC (version 0.11.5)