FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_GR_Dam_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_GR_Dam_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29094822
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1197800.067.06274412
GAGGATC3661700.066.89606511
AGGATCA1065150.066.8551312
AGGATCG478850.066.16650412
GGATCAA242350.065.76617413
GGATCTC396100.065.6856213
GGATCTT329300.065.6824513
CCGAGGA3745000.065.612999
GGATCTA110400.065.59096513
GGATCCA310550.065.4219213
CGAGGAT3754100.065.409910
GGTCGCG3778600.065.053811
GGATCCC195350.064.9452213
CGGCCGA3859950.064.749646
GGCCGAG3864600.064.7110447
GGATCTG397800.064.4811113
GGATCAT319450.064.4428513
GGATCCG172350.064.3522213
GGATCGT129050.064.1395213
GCCGAGG3921550.063.7542238
Produced by FastQC (version 0.11.5)