FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_bZIP1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908232
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT578250.066.2985112
GAGGATC1902850.066.1534611
AGGATCA576150.065.8658812
GGTCGCG1964650.065.807321
CCGAGGA1927650.065.5227669
CGAGGAT1933450.065.2295610
AGGATCG282800.065.2195612
GGATCTA46600.065.1150513
CGGCCGA2043500.064.4297566
GGATCTT160600.064.28722413
GGATCTC189700.064.2221613
GGCCGAG2055050.064.070357
GCCGAGG2062850.063.7602168
GGATCAA125650.063.72968313
GGATCCA153900.063.72028413
GTCGCGG2047400.063.403732
GGATCAT159700.062.9839713
GGATCCC102400.062.64850213
GGATCTG206100.062.4061713
CGCGGCC2108400.062.0869984
Produced by FastQC (version 0.11.5)