FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_bZIP1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12979058
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1650850.065.860841
AGGATCT498850.065.5546212
GAGGATC1610850.065.5484411
CCGAGGA1626900.065.11319
AGGATCA482850.064.89284512
AGGATCG238650.064.7455212
CGAGGAT1635450.064.70396410
CGGCCGA1732200.063.9307336
GGCCGAG1744150.063.523557
GGATCTT139550.063.3498613
GCCGAGG1753050.063.0949948
GTCGCGG1730800.063.090812
GGATCTA43800.063.0441813
GGATCAA110000.062.99624313
GGATCCA135550.062.74062713
GGATCTC163900.062.6716713
GGATCCC84400.061.9512813
TCGCGGC1778300.061.509453
CGCGGCC1794500.061.410844
GGATCCG87300.061.37663713
Produced by FastQC (version 0.11.5)