FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_bZIP1_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_bZIP1_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24351382
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1009600.066.8763712
GAGGATC3223850.066.7736611
AGGATCA1013750.066.6647412
CGAGGAT3285350.065.6495310
GGATCTA94800.065.6039813
CCGAGGA3294850.065.565549
AGGATCG420750.065.47124512
GGATCAA247600.065.4316113
GGTCGCG3337200.065.3720861
GGATCTC316250.065.3381613
GGATCTT284000.065.30202513
GGATCCA268600.065.16312413
CGGCCGA3438350.064.594126
GGATCAT295650.064.5400113
GGCCGAG3449450.064.4190447
GGATCCC165500.064.3298113
GGATCTG347200.064.1507213
GCCGAGG3474900.063.8999028
GTCGCGG3438500.063.6646842
GGATCAC241250.063.38198513
Produced by FastQC (version 0.11.5)