FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15290981
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA221410.14479777327563223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT589550.066.9150912
GAGGATC1847500.066.88389611
AGGATCA575900.066.7448312
AGGATCG244850.066.3083212
CGAGGAT1874950.066.0059110
GGATCAA155550.065.4968213
GGTCGCG1919650.065.465941
CCGAGGA1897750.065.330939
GGATCTC173700.065.14091513
GGATCCA157050.064.8935313
CGGCCGA1974600.064.858686
GGATCTT185800.064.7790313
GGCCGAG1986000.064.514827
GCCGAGG1997650.064.092818
GGATCAT170100.064.091713
GGATCTA66550.064.05414613
GTCGCGG1975950.063.874112
GGATCTG186650.063.80913
GGATCCC89100.063.5548713
GGATCCG91750.063.1687513
Produced by FastQC (version 0.11.5)