FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16520934
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT586550.066.4096812
GAGGATC1845500.065.7747311
AGGATCA529350.065.7246712
GGTCGCG1897950.065.6088261
CCGAGGA1871800.064.9672559
AGGATCG277450.064.9500412
GGATCTA56600.064.92650613
CGAGGAT1872350.064.9006610
GGATCTT185050.064.3419713
CGGCCGA1974300.064.1831746
GGATCTC189250.063.65376713
GGCCGAG1992250.063.644627
GGATCAA127000.063.46574413
GCCGAGG1995400.063.4566428
GGATCCA148800.063.2230613
GTCGCGG1986600.062.928712
CGCGGCC2029400.062.093674
GGATCTG183950.062.04405213
GGATCAC123950.062.00617213
GGATCCC96800.061.89813613
Produced by FastQC (version 0.11.5)