FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_GR_Dam_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_GR_Dam_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26360061
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT988100.066.5175212
GAGGATC3191850.066.1937511
AGGATCA975500.065.9308512
GGTCGCG3301600.065.4529341
CCGAGGA3255800.065.169849
AGGATCG446400.065.1348912
CGAGGAT3252950.065.10499610
GGATCTA90000.065.0964913
GGATCTC316400.064.9301213
GGATCAA236800.064.6016713
CGGCCGA3424600.064.371466
GGATCTT277900.064.2660513
GGCCGAG3445500.063.984537
GCCGAGG3469850.063.4870958
GGATCCA267900.063.3597813
GTCGCGG3426200.063.353572
GGATCCC173900.063.093213
GGATCTG345600.062.46190613
GGATCAT276650.062.34255613
CGCGGCC3524150.062.172914
Produced by FastQC (version 0.11.5)