FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_GR_Only_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_GR_Only_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25882324
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1088750.067.2151212
AGGATCA1006800.067.1799812
GAGGATC3417400.067.1102411
GGATCAA219250.066.5799313
AGGATCG481750.066.3705512
GGATCTA99050.066.3565613
GGATCTC357350.066.3526513
CCGAGGA3481150.066.098549
GGATCTT279550.065.9646813
GGATCCA279050.065.9198313
CGAGGAT3501000.065.6766710
GGATCAT276050.065.6473213
GGATCTG375400.065.4743413
GGATCAG296200.065.3641913
GGATCCG166150.065.2986413
GGTCGCG3518250.065.275741
GGATCCC181200.065.12865413
CGGCCGA3597450.064.970886
GGCCGAG3598900.064.96087
GGATCAC241900.064.7874713
Produced by FastQC (version 0.11.5)