FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_GR_Only_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_GR_Only_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26366392
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA372630.14132764164319486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1016200.066.66176612
AGGATCA918150.066.6221312
AGGATCG422350.066.4986712
GAGGATC3156250.066.4353111
GGATCTC332100.065.92863513
GGATCCA256750.065.75716413
GGATCAA212300.065.7432813
GGATCTT270150.065.5787813
GGATCTA95700.065.2422713
GGATCTG338300.065.0824413
CGAGGAT3238100.064.9389210
GGATCCC166050.064.8958613
GGATCCG160350.064.8018613
GGATCAC211500.064.7839713
GGATCGT111450.064.75223513
CCGAGGA3252400.064.688919
GGATCAT268950.064.6611813
GGATCAG251600.064.474113
GGTCGCG3282750.064.090331
CGGCCGA3353650.063.806536
Produced by FastQC (version 0.11.5)